05:- To find BLOOD GROUP
Aim :- To find blood group
Requirement :- Lancet, cotton, slides, magnifine glass, Spirit, Anti A,B,D
Procedure :- First we have to take blood sample from the person s’ left hand from fourth finger. Before that we have to put spirit on cotton and apply to the person s’ finger, then by the help of lancet we have to prick a hole on the finger and take three drops on the slide.
on one side of drop we have to add one drop of antic-era A and on second antic-era B and on third antic-era D. After mixing them we checked the blood group of the person and applied spirit to the finger to stop the blood flow.
Observation :- In the first two antic-era A or B if the blood gets in small crystal form, we can also know about blood group by following table.
|1.||If the blood get crystallized in antic-era A and not in antic-era B then||Blood group is “A”|
|2.||If the blood get crystallized in antic-era B and not in antic-era A then||Blood group is “B”|
|3.||If the blood get crystallized in antic-era A and B then||Blood group is “AB”|
|4.||If the blood not get crystallized in antic-era A and B then||Blood group is “O”|
For the third one you have to go through “RH” factor.
|1.||If the blood get crystallized in antic-era “D” then||RH factor is “Positive”|
|2.||If the blood not get crystallized in antic-era “D” then||RH factor is “Negative”|
Objective:- Take blood sample of every one in the laboratory and show to your guide or teacher. Visit pathologist or government hospital. Do blood test by help of the doctor.
Important information:- Blood :- It is a genetical liquid form made by the red blood cells (R.B.C.) and white blood cells (W.B.C.) and platelets , thrombosis its’ all use in formation of blood.
Antigens :- Anti means which does the opposite reaction to the diseases. Gen means which grows or produces. It means body that produces unwanted bacteria or any other thing in the body to stop it antigen start to work and try to kill it.
- Before practical remove antic-era A,B,D from refrigerator .
- We have to keep temperature of antic-era upto 2-6 degree
- If you are not able to identify the blood group then, give the sample to laboratory.
- Use on Pro for one person.
- Prick lancet on the tip of finger not beside the nail.
- To prick lancet take middle finger or the fourth finger.